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| Kruger,Ernesto Renato; Penha,Tania Regina; Stoffelo,Daura Regina Eira; Roehe,Paulo Michel; Ribeiro,Magda Costa; Soccol,Vanete Thomaz. |
| Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinaesub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoHV-4 virus; PCR; RFLP; Fingerprint. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100279 |
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| Radmann,Elizete Beatriz; Bianchi,Valmor João; Oliveira,Roberto P. de; Fachinello,José Carlos. |
| O objetivo deste trabalho foi caracterizar a diversidade genético-molecular, por marcadores RAPD, das dez principais cultivares de morangueiro utilizadas no País: Aromas, Bürkley, Camarosa, Campinas, Dover, Milsei-Tudla, Oso Grande, Santa Clara, Sweet Charlie e Vila Nova. O DNA foi extraído de folhas maduras, as análises RAPD foram realizadas com 26 primers e os produtos de amplificação separados por eletroforese. O coeficiente de Dice foi utilizado para estimar a similaridade genética entre as cultivares e o método UPGMA para gerar o fenograma por meio do NTSYS. Houve amplificação de fragmentos consistentes com 19 primers, tendo sido encontrado polimorfismo em 14. Dos 116 fragmentos gerados, 84 foram polimórficos. As cultivares foram classificadas em dois... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fragaria x ananassa Duch.; RAPD; Fingerprint; Marcador molecular. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362006000100017 |
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| Santos,Leonardo Silva; Soriano,Maria del Pilar Camarantin; Mirabal-Gallardo,Yaneris; Carrasco-Sanchez,Veronica; Nachtigall,Fabiane Manke; Pereira,Iris; Pereira,Eugenia. |
| The aim of this work was to study a fast, new, sensitive, and simple method for the chemotaxonomic classification of Chilean lichens (Teloschistes chrysophthalmus, Ramalina farinacea, Usnea pusilla, Ramalina chilensis and Stereocaulon ramulosum) using MALDI-TOF-MS and UPLC-ESI(-)-MS data. Lichens soluble proteins fingerprints were acquired by MALDI-TOF-MS and they were analyzed by chemometric (PCA). Lichens organic extracts fingerprints were obtained by UPLC-ESI(-)-MS. MALDI-TOF-MS associated with chemometric analysis was used to detect new m/z patterns of soluble proteins that were compared with Protein Data Bank of UnitPro. These data also permitted the satisfactory distinction among the families and species. UPLC-ESI(-)-MS fingerprints analyses of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chilean lichens; Chemotaxonomic; Fingerprint; MALDI-TOF-MS; UPLC-ESI(-)-MS. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200244 |
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| Haffray, Pierrick; Vandeputte, Marc; Petit, V.; Pincent, Cedric; Chatain, Beatrice; Chapuis, H.; Meriaux, J. C.; Coudurier, B.; Quillet, E.; Dupont-nivet, M.. |
| Application of DNA parentage assignment for commercial selection in aquaculture is still rare. An experiment was performed to quantify and to minimize maternal effects on growth in rainbow trout. Six hundred families were reared until 198 day post fertilization (dpf) according to two different procedures. In the first procedure (NORM), all families were pooled at eyed stage. In the second procedure (MIN), where maternal effects due to differences in egg size were expected to be minimal, the spawns were divided into sub-groups with similar mean egg weight at eyed stage. These sub-groups were then pooled when they achieved the same mean body length (147 dpf). Genetic parameters were estimated for body weight, body length and condition factor at 198 dpf, with... |
| Tipo: Text |
Palavras-chave: Rainbow trout; Fingerprint; Maternal effect; Heritability; Growth; Triploid. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00115/22639/20395.pdf |
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| Andrade,M.K.N.; Machado,S.M.A.; Leite,M.L.; Saad,M.H.F.. |
| Assuming that the IS6110-restriction fragment length polymorphism (RFLP) changes at a constant rate of 3.2 years, this methodology was applied to demonstrate, for the first time, variant patterns of Mycobacterium tuberculosis (MTB) in multiple isolates obtained at short time intervals from sputum and blood of an HIV+ patient with multiple admissions to the Emergency Room and to the multidrug-resistant tuberculosis (MDR-TB) Reference Center of a secondary-care hospital in Rio de Janeiro, Brazil. In sputum, the IS6110-RFLP appeared in isolates with two variant patterns with 10 and 13 IS6110 copies. However, blood presented only the pattern corresponding to 10 copies, suggesting compartmentalization. With regard to the exact match of 10 of 13 bands, this may... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Tuberculosis; Mycobacterium tuberculosis; TB-MDR; TB-RFLP; Gene mutation; Fingerprint. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500006 |
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